DNA helicase (5’-3’) that  may help  to promote replisome progression in some regions of the genome such as the rRNA genes. Related to Pif1.




Molecular weight

82 kDa (Sc)

Biochemical properties




Protein interactions


•Pcna (1)

•Pole on chromosomes (2)

•DNA2 Synthetic lethal (3)






Cellular location and expression


•possible role in rDNA replication more recombination ( popped out ribosomal circles) if lost (4) (opposing effect to pif )

•Suggested to help Mcm2-7helicase pass rDNA region (5)

•More pause sites in telomeres if k/o (6)

• role in replication tRNA genes and mating type loci (needed in regions of odd structure?) (7)

•Mutants accumulate at pause sites but not origins (8)

•Not essential but is in mrc1 mutant cells (9)

•Suggested to move with replication fork and affect general replication all chromosomes (2)


Other comments


Revised by


Last edited

14 April 08



1.    Schmidt,K.H., Derry,K.L. and Kolodner,R.D. (2002) Saccharomyces cerevisiae RRM3, a 5' to 3' DNA helicase, physically interacts with proliferating cell nuclear antigen. J Biol Chem 277, 45331-45337.

2.    Azvolinsky,A., Dunaway,S., Torres,J.Z., Bessler,J.B. and Zakian,V.A. (2006) The S. cerevisiae Rrm3p DNA helicase moves with the replication fork and affects replication of all yeast chromosomes. Genes Dev 20, 3104-3116.

3.    Weitao,T., Budd,M., Hoopes,L.L. and Campbell,J.L. (2003) Dna2 helicase/nuclease causes replicative fork stalling and double-strand breaks in the ribosomal DNA of Saccharomyces cerevisiae. J Biol Chem 278, 22513-22522.

4.    Ivessa,A.S., Zhou,J.Q. and Zakian,V.A. (2000) The Saccharomyces Pif1p DNA helicase and the highly related Rrm3p have opposite effects on replication fork progression in ribosomal DNA. Cell 100, 479-489.

5.    Bessler,J.B., Torredagger,J.Z. and Zakian,V.A. (2001) The Pif1p subfamily of helicases: region-specific DNA helicases? Trends Cell Biol 11, 60-65.

6.    Ivessa,A.S., Zhou,J.Q., Schulz,V.P., Monson,E.K. and Zakian,V.A. (2002) Saccharomyces Rrm3p, a 5' to 3' DNA helicase that promotes replication fork progression through telomeric and subtelomeric DNA. Genes Dev 16, 1383-1396.

7.    Ivessa,A.S., Lenzmeier,B.A., Bessler,J.B., Goudsouzian,L.K., Schnakenberg,S.L. and Zakian,V.A. (2003) The Saccharomyces cerevisiae helicase Rrm3p facilitates replication past nonhistone protein-DNA complexes. Mol Cell 12, 1525-1536.

8.    Calzada,A., Hodgson,B., Kanemaki,M., Bueno,A. and Labib,K. (2005) Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork. Genes Dev 19, 1905-1919.

9.    Szyjka,S.J., Viggiani,C.J. and Aparicio,O.M. (2005) Mrc1 is required for normal progression of replication forks throughout chromatin in S. cerevisiae. Mol Cell 19, 691-697.